Thermolabile Uracil-DNA Glycosylase removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme ( 1-10 units) is completely inactivated by a 10 minute incubation at temperatures greater than 50°C in the 1x reaction buffer as measured in the unit characterization assay.

Source of ProteinA recombinant E. coli strain carrying the recombinant thermolabile Uracil DNA Glycosylase gene isolated from a marine bacteria.

Supplied in50 mM Tris-HCl50 mM NaCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C

Supplied With:B5020 (10X Reaction Buffer)

10X Reaction Buffer (B5020):700 mM Tris-HCl100 mM NaCl10 mM EDTA1 mg/mL BSApH 8.0 @ 25°C

Unit Definition1 unit is defined as the amount of Thermolabile UDG required to release 1nmol of Uracil from dU-containing DNA in one hour at 37°C.

Enzymatics常用产品报价单