T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded nucleic acids (RNA or DNA).
Source of ProteinA recombinant E. coli strain carrying the T4 RNA Ligase gene from bacteriophage T4.
Supplied in10 mM Tris-HCl50 mM KCl0.1 mM EDTA1.0 mM DTT50% glycerolpH 7.5 @ 25°C
Supplied With B6050 (10X T4 RNA Ligase Buffer)
10X T4 RNA Ligase Buffer (B6050)500 mM Tris-HCl100 mM MgCl210 mM ATP100 mM DTTpH 7.8 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme required to ligate 50% of 0.4 µg of an equimolar mix of two single stranded 23 base RNA oligonucleotides (one 5′-phosphorylated) in 20 µL 1X T4 RNA Ligase Buffer following a 30 minute incubation at 37°C.
Enzymatics常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
Enzymatics/T4 Gene 32 Protein/Y9130L/1.0 mg | Y9130L | Binding Proteins | 测序酶 | 371.00 |