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主营:连接蛋白、DNA修饰酶、NGS文库制备(NGS Library Construction)、核酸和RNA酶。
℡ 4000-520-616
℡ 4000-520-616
Enzymatics/T4 DNA Ligase (Rapid)/240,000 U/L6030-HC-L
产品编号:L6030-HC-L
市  场 价:¥10480.00
场      地:美国(厂家直采)
产品分类: 分子类>二代测序>测序酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$524.00
品      牌: Enzymatics
公      司:Enzymatics,inc
公司分类:
Enzymatics/T4 DNA Ligase (Rapid)/240,000 U/L6030-HC-L
商品介绍

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).

Source of ProteinA recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.

Supplied In10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C

Supplied WithB1010 (2X Rapid Ligation Buffer)B6030 (10X T4 DNA Ligase Buffer)

2X Rapid Ligation Buffer (B1010):132mM Tris-HCI20 mM MgCl22 mM DTT2 mM ATP15% PEG 6000pH 7.6 @ 25°C

10X T4 DNA Ligase Buffer (B6030):500mM Tris-HCI100 mM MgCl250 mM DTT10 mM ATPpH 7.6 @ 25°C

Unit Definition1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C.

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产品名货号公司分类商城分类美金价
Enzymatics/T4 Gene 32 Protein/Y9130L/1.0 mgY9130LBinding Proteins测序酶371.00
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