T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).

Source of ProteinA recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.

Supplied In10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C

Supplied WithB1010 (2X Rapid Ligation Buffer)B6030 (10X T4 DNA Ligase Buffer)

2X Rapid Ligation Buffer (B1010):132mM Tris-HCI20 mM MgCl₂2 mM DTT2 mM ATP15% PEG 6000pH 7.6 @ 25°C

10X T4 DNA Ligase Buffer (B6030):500mM Tris-HCI100 mM MgCl₂50 mM DTT10 mM ATPpH 7.6 @ 25°C

Unit Definition1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C.

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