T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA. In the absence of 20-30% PEG 6000, T3 DNA Ligase displays a very low efficiency for blunt-ended ligation. (1) T3 DNA Ligase displays a higher efficiency for joining A/T overhangs than C/G matched ends. (1) T3 DNA Ligase retains 95% of its activity in 1.0 M NaCl or KCl, with an optimal concentration of 300 mM(1).

Source of ProteinA recombinant E. coli strain carrying the T3 DNA Ligase gene.

Supplied in20 mM Tris-HCl300 mM NaCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C

Supplied With:B1010 (2X Rapid Ligation Buffer)

2X Rapid Ligation Buffer (B1010):132mM Tris-HCI20 mM MgCl₂2 mM DTT2 mM ATP15% PEG 6000pH 7.6 @ 25°C

Unit Definition1 unit is defined as the amount of T3 DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 23°C.

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