T7 DNA Polymerase is the mesophilic, highly processive, replicative DNA polymerase from bacteriophage T7 that is responsible for the rapid and accurate replication of the virus’ genome during its infection cycle. T7 DNA Polymerase is a two subunit protein, consisting of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (E. coli trxA gene thioredoxin) (1,2). The enzyme possesses a powerful (3’→5′) nuclease activity that acts on both single and double stranded DNA and appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis (3,4,5).

Source of ProteinA recombinant E. coli strain carrying the bacteriophage T7 gene 5.

Supplied in50 mM KPO₄0.1 mM EDTA1.0 mM DTT50% glycerolpH 7.0 @ 25°C

Supplied WithB7260 (10X T7 DNA Polymerase Buffer)10X T7 DNA Polymerase Buffer (B7260)400 mM Tris-HCl200 mM MgCl₂500 mM NaClpH 7.5 @ 25°C

Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of total dNTPs into acid insoluble material in 30 minutes at 37°C.

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