M-MuLV Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate and exhibits no measurable proofreading 3′→5′ exonuclease function.  This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template.

Source of ProteinA recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

Supplied in50 mM Tris-HCl150 mM NaCl0.1 mM EDTA1 mM DTT0.1% NP-40 Alternative50% glycerolpH 7.6 @ 25°C

Supplied WithB7040 (10X M MuLV RT Buffer)

10X M-MuLV RT Buffer (B7040):500 mM Tris-HCl750 mM KCl30 mM MgCl₂100 mM DTTpH 8.3 @ 25°C

Unit Definition1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

Enzymatics常用产品报价单