Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′→5′) nuclease activities, and, in the absence of the holoenzyme’s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.

Source of ProteinA recombinant E. coli strain carrying the Klenow Fragment gene.

Supplied in 100 mM KPO₄1.0 mM DTT0.1mM EDTA50% glycerolpH 7.4 @ 25°C

Supplied WithB0110 (10X Blue Buffer)

10X Blue Buffer (B0110)500 mM NaCl100 mM Tris-HCl100 mM MgCl₂10 mM DTTpH 7.9 @ 25°C

Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.

Enzymatics常用产品报价单