Klenow (3′→ 5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′→ 5′) and nick-translation (5′→ 3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.
Source of ProteinA recombinant E. coli strain carrying the Klenow (3′→5′ exo-) gene.
Supplied in 20 mM Tris-HCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C
Supplied WithB0110 (10X Blue Buffer)10X Blue Buffer (B0110)500mM NaCl100 mM Tris-HCl100 mM MgCl210 mM DTTpH 7.9 @ 25°C
Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble materialin 30 minutes at 37°C.
Enzymatics常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| Enzymatics/T4 Gene 32 Protein/Y9130L/1.0 mg | Y9130L | Binding Proteins | 测序酶 | 371.00 |
本网站将在规定时间内给予删除等相关处理。
